Bio-analytical services for life science and drug discovery communities
BioAssay Systems develops high-quality, innovative assay kits and offers bio-analytical services to life science and drug discovery communities. Our assays can be used with a wide variety of samples and have been frequently cited in papers published in first-tier journals.
§ Blood/Urine Chemistry § Enzyme Activity Assays § Metabolism Assays § Oxidative Stress Assays § Cation and Anion Assays § High-throughput Assays
Cost-Effective, Sensitive and Convenient Dual Luciferase Reporter Gene Assays The accuracy of reporter assays can be improved by utilizing a dual reporter system. One of the reporter genes is correlated with the promoter of interest and is used to assess the effects of specific experimental conditions, while the second reporter is used as a control and serves as a baseline response. The Super Light™ Dual- Luciferase Reporter Assay allows for the sequential measurement of the activity of two different luciferases, firefly (FFL) and Renilla (RL), in a single sample. The firefly luciferase luminescence is measured first by addition of the FFL Reagent. Next, the RL Reagent is added to the same well. The RL Reagent simultaneously quenches the firefly luciferase luminescence and initiates the Renilla luciferase reaction. The light production of both reactions can be conveniently measured on a luminometer.
BioAssay Systems' bioluminescent dual reporter gene assay is extremely sensitive and is especially suitable for quantifying dual luciferase expression in recombinant cells or in cell free transcription/translation reactions. Assays can be performed in tubes, cuvettes or multi-well plates. KEY FEATURES High sensitivity and wide detection range. Detection of as little of 2 fg luciferase. Compatible with routine laboratory and HTS formats. Assays can be performed in tubes or microplates, and measured with any luminometer. Can be readily automated on HTS liquid handling systems. Fast and convenient. Three step assay allows detection of dual luciferase levels within 20 minutes. Cost-effective. Only $1.19/well of assays in 96-well plate, $119 for this 100-assay kit. APPLICATIONS Gene Regulation. gene expression level, characterization of promoter activity, modulation of gene expression by receptors, transcription factors and small molecules.
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